dapi fluoromount g southernbiotech cat (SouthernBiotech)
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SouthernBiotech
dapi fluoromount g southernbiotech cat
Dapi Fluoromount G Southernbiotech Cat, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 4648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/southernbiotech+cat/DAPI+Fluoromount-G/pm41856110-301-47-49
Average 96 stars, based on 4648 article reviews
Dapi Fluoromount G Southernbiotech Cat, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 4648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/southernbiotech+cat/DAPI+Fluoromount-G/pm41856110-301-47-49
Average 96 stars, based on 4648 article reviews
dapi fluoromount g southernbiotech cat - by Bioz Stars,
2026-09
96/100 stars
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other:Article Title: Treatment of experimental asthma using a novel peptide inhibitor of the inducible T cell kinase Article Snippet: -!mouse IgG2a, clone HOPC-1 - Article Title: Kinase-dead ATR differs from ATR loss by limiting the dynamic exchange of ATR and RPA Article Snippet: Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Western Blot: rabbit polyclonal pKAP1 (S824), A300-767A, Bethyl Laboratories rabbit monoclonal KAP1 (TIF1β), C42G12, Cell Signaling, #4124 rabbit monoclonal pCHK1 Ser245 (133D3), Cell Signaling, #2348 mouse monoclonal CHK1 (2G1D5), Cell Signaling, #2360 rabbit monoclonal anti-RPA32/RPA2 (phospho T21), Abcam, ab109394 rabbit polyclonal phospho RPA32 (S4/S8), A300-245A, Bethyl Laboratories rabbit polyclonal RPA32 antibody, A300-244A, Bethyl Laboratories rabbit polyclonal anti phospho H2AX (Ser139), 07-164, Millipore rabbit polyclonal anti-histone H2AX, 07-627, Millip2ore rabbit polyclonal ATR antibody, Cell Signaling, #2790 mouse monoclonal anti-vinculin antibody, clone V284, 05-386, Millipore 3 nature research | reporting sum m ary April 2018 mouse monoclonal anti-β-actin antibody, clone AC-15, A1978, Sigma mouse monoclonal anti α-tubulin (DM1A), CP06, Millipore Immunofluorescence: rabbit polyclonal anti-Rad51 (Ab-1), PC-130, Calbiochem rabbit polyclonal anti-SCP3, Abcam, ab15093 mouse monoclonal anti-SCP3 [Cor 10G11/7], Abcam, ab97672 mouse anti-human MLH1, clone G168-15, BD Pharmingen, cat. No. 550838 rat monoclonal anti-BrdU antibody [BU1/75 (ICR1)], Abcam, ab6326 mouse anti-BrdU, clone B44, Beckton Dickinson, cat. No. 347580 anti-DNA antibody, single-stranded, clone 16-19, MAB3034, Millipore FACS analysis: FITC-conjugated mouse anti-BrdU antibody, BD Pharmingen, cat. No. 556028 mouse monoclonal anti-Replication Protein A (Ab-3), RPA34-20, NA19L, Millipore FITC rat anti-mouse IgG1, clone A85-1, BD Pharmingen, cat. No. 553443 PE-Cyanine5 rat anti-mouse CD45R/B220, clone RA3-6B2, BD Pharmingen, cat. No. 553091 FITC rat anti-Cd11b, clone M1/70, BD Pharmingen, cat. No. 553310 APC anti-mouse Ly-6G/Ly-6C (Gr-1), clone RB6-8C5, BioLegend Goat anti-mouse IgM, Human ads-PE, Control:Article Title: Renal protection induced by physical exercise may be mediated by the irisin/AMPK axis in diabetic nephropathy Article Snippet: .. Groups: DM, DM+CycloRGDyK DM+Exe and DM+Exe+CycloRGDyK Begining of CycloRGDyK treatment (1mg/kg) A. pAMPK(Trh172) Vinculin AMPKa Vinculin pACC(Ser79) Vinculin Vinculin ACC Vinculin Vinculin Vinculin pNF-kB(p65) NF-kB(p65) Vinculin Collagen IV Fibronectin NG (24h) HG (24h) NG (48h) HG (48h) B. NG (24h) HG (24h) NG (48h) HG (48h) Parameters Non diabetic control Sedentary diabetics Exercised diabetics Gender (male/female) 6/9 9/6 7/8 Age (years) 47.0 ± 4.8 51.7 ± 4.5 50.6 ± 3.9 BMI (weight/height2) 26.8 ± 3.3 30.6 ± 3.1# 29.4 ± 3.6# Diabetes diagnosis (years) - 4.4 ± 2.0 5.5 ± 2.5 Fasting glucose (mg/dL) 84.9 ± 8.7 144.7 ± 40.8# 131.8 ± 30.9# Glycated hemoglobin (%) NA 7.3 ± 1.3 7.2 ± 1.5 Systolic blood pressure (mmHg) NA 123.3 ± 12.9 113.0 ± 14.6 Diastolic blood pressure (mmHg) NA 79.1 ± 7.4 73.2 ± 9.0 Serum irisin (mg/mL) NA 1.1 ± 0.5 1.7 ± 0.6* Supplementary Table 2 Antibodies (dilution) Source Identifier Anti-rabbit phospho-AMPKa Thr172 (1:1000) Cell Signaling Technology Cat. # 2535 Anti-rabbit AMPKa (1:1000) Cell Signaling Technology Cat. # 5831 Anti-rabbit phospo-ACC Ser79 (1:1000) Cell Signaling Technology Cat. # 11818 Anti-rabbit ACC (1:1000) Cell Signaling Technology Cat. # 3676 Anti-rabbit vinculin (1:1000) Cell Signaling Technology Cat. # 13901 Anti-rabbit GAPDH (1:1000) Cell Signaling Technology Cat. # 5174 Anti-rabbit phopho -NF-kB p65 (1:1000) Cell Signaling Technology Cat. # 3033S Anti-rabbit PGC1 -a (1:250) Cell Signaling Technology Cat. # 2178S Anti-rabbit fibronectin (1:100 – WB, 1:50 – IHC) Abcam Cat. # ab2413 Anti-rabbit type IV collagen (1:50 – IHC) Abcam Cat. # ab6586 Anti-goat type IV collagen (1:1000) Biomarker Discovery:Article Title: Renal protection induced by physical exercise may be mediated by the irisin/AMPK axis in diabetic nephropathy Article Snippet: .. Groups: DM, DM+CycloRGDyK DM+Exe and DM+Exe+CycloRGDyK Begining of CycloRGDyK treatment (1mg/kg) A. pAMPK(Trh172) Vinculin AMPKa Vinculin pACC(Ser79) Vinculin Vinculin ACC Vinculin Vinculin Vinculin pNF-kB(p65) NF-kB(p65) Vinculin Collagen IV Fibronectin NG (24h) HG (24h) NG (48h) HG (48h) B. NG (24h) HG (24h) NG (48h) HG (48h) Parameters Non diabetic control Sedentary diabetics Exercised diabetics Gender (male/female) 6/9 9/6 7/8 Age (years) 47.0 ± 4.8 51.7 ± 4.5 50.6 ± 3.9 BMI (weight/height2) 26.8 ± 3.3 30.6 ± 3.1# 29.4 ± 3.6# Diabetes diagnosis (years) - 4.4 ± 2.0 5.5 ± 2.5 Fasting glucose (mg/dL) 84.9 ± 8.7 144.7 ± 40.8# 131.8 ± 30.9# Glycated hemoglobin (%) NA 7.3 ± 1.3 7.2 ± 1.5 Systolic blood pressure (mmHg) NA 123.3 ± 12.9 113.0 ± 14.6 Diastolic blood pressure (mmHg) NA 79.1 ± 7.4 73.2 ± 9.0 Serum irisin (mg/mL) NA 1.1 ± 0.5 1.7 ± 0.6* Supplementary Table 2 Antibodies (dilution) Source Identifier Anti-rabbit phospho-AMPKa Thr172 (1:1000) Cell Signaling Technology Cat. # 2535 Anti-rabbit AMPKa (1:1000) Cell Signaling Technology Cat. # 5831 Anti-rabbit phospo-ACC Ser79 (1:1000) Cell Signaling Technology Cat. # 11818 Anti-rabbit ACC (1:1000) Cell Signaling Technology Cat. # 3676 Anti-rabbit vinculin (1:1000) Cell Signaling Technology Cat. # 13901 Anti-rabbit GAPDH (1:1000) Cell Signaling Technology Cat. # 5174 Anti-rabbit phopho -NF-kB p65 (1:1000) Cell Signaling Technology Cat. # 3033S Anti-rabbit PGC1 -a (1:250) Cell Signaling Technology Cat. # 2178S Anti-rabbit fibronectin (1:100 – WB, 1:50 – IHC) Abcam Cat. # ab2413 Anti-rabbit type IV collagen (1:50 – IHC) Abcam Cat. # ab6586 Anti-goat type IV collagen (1:1000) Western Blot:Article Title: Renal protection induced by physical exercise may be mediated by the irisin/AMPK axis in diabetic nephropathy Article Snippet: .. Groups: DM, DM+CycloRGDyK DM+Exe and DM+Exe+CycloRGDyK Begining of CycloRGDyK treatment (1mg/kg) A. pAMPK(Trh172) Vinculin AMPKa Vinculin pACC(Ser79) Vinculin Vinculin ACC Vinculin Vinculin Vinculin pNF-kB(p65) NF-kB(p65) Vinculin Collagen IV Fibronectin NG (24h) HG (24h) NG (48h) HG (48h) B. NG (24h) HG (24h) NG (48h) HG (48h) Parameters Non diabetic control Sedentary diabetics Exercised diabetics Gender (male/female) 6/9 9/6 7/8 Age (years) 47.0 ± 4.8 51.7 ± 4.5 50.6 ± 3.9 BMI (weight/height2) 26.8 ± 3.3 30.6 ± 3.1# 29.4 ± 3.6# Diabetes diagnosis (years) - 4.4 ± 2.0 5.5 ± 2.5 Fasting glucose (mg/dL) 84.9 ± 8.7 144.7 ± 40.8# 131.8 ± 30.9# Glycated hemoglobin (%) NA 7.3 ± 1.3 7.2 ± 1.5 Systolic blood pressure (mmHg) NA 123.3 ± 12.9 113.0 ± 14.6 Diastolic blood pressure (mmHg) NA 79.1 ± 7.4 73.2 ± 9.0 Serum irisin (mg/mL) NA 1.1 ± 0.5 1.7 ± 0.6* Supplementary Table 2 Antibodies (dilution) Source Identifier Anti-rabbit phospho-AMPKa Thr172 (1:1000) Cell Signaling Technology Cat. # 2535 Anti-rabbit AMPKa (1:1000) Cell Signaling Technology Cat. # 5831 Anti-rabbit phospo-ACC Ser79 (1:1000) Cell Signaling Technology Cat. # 11818 Anti-rabbit ACC (1:1000) Cell Signaling Technology Cat. # 3676 Anti-rabbit vinculin (1:1000) Cell Signaling Technology Cat. # 13901 Anti-rabbit GAPDH (1:1000) Cell Signaling Technology Cat. # 5174 Anti-rabbit phopho -NF-kB p65 (1:1000) Cell Signaling Technology Cat. # 3033S Anti-rabbit PGC1 -a (1:250) Cell Signaling Technology Cat. # 2178S Anti-rabbit fibronectin (1:100 – WB, 1:50 – IHC) Abcam Cat. # ab2413 Anti-rabbit type IV collagen (1:50 – IHC) Abcam Cat. # ab6586 Anti-goat type IV collagen (1:1000) Immunohistochemistry:Article Title: Renal protection induced by physical exercise may be mediated by the irisin/AMPK axis in diabetic nephropathy Article Snippet: .. Groups: DM, DM+CycloRGDyK DM+Exe and DM+Exe+CycloRGDyK Begining of CycloRGDyK treatment (1mg/kg) A. pAMPK(Trh172) Vinculin AMPKa Vinculin pACC(Ser79) Vinculin Vinculin ACC Vinculin Vinculin Vinculin pNF-kB(p65) NF-kB(p65) Vinculin Collagen IV Fibronectin NG (24h) HG (24h) NG (48h) HG (48h) B. NG (24h) HG (24h) NG (48h) HG (48h) Parameters Non diabetic control Sedentary diabetics Exercised diabetics Gender (male/female) 6/9 9/6 7/8 Age (years) 47.0 ± 4.8 51.7 ± 4.5 50.6 ± 3.9 BMI (weight/height2) 26.8 ± 3.3 30.6 ± 3.1# 29.4 ± 3.6# Diabetes diagnosis (years) - 4.4 ± 2.0 5.5 ± 2.5 Fasting glucose (mg/dL) 84.9 ± 8.7 144.7 ± 40.8# 131.8 ± 30.9# Glycated hemoglobin (%) NA 7.3 ± 1.3 7.2 ± 1.5 Systolic blood pressure (mmHg) NA 123.3 ± 12.9 113.0 ± 14.6 Diastolic blood pressure (mmHg) NA 79.1 ± 7.4 73.2 ± 9.0 Serum irisin (mg/mL) NA 1.1 ± 0.5 1.7 ± 0.6* Supplementary Table 2 Antibodies (dilution) Source Identifier Anti-rabbit phospho-AMPKa Thr172 (1:1000) Cell Signaling Technology Cat. # 2535 Anti-rabbit AMPKa (1:1000) Cell Signaling Technology Cat. # 5831 Anti-rabbit phospo-ACC Ser79 (1:1000) Cell Signaling Technology Cat. # 11818 Anti-rabbit ACC (1:1000) Cell Signaling Technology Cat. # 3676 Anti-rabbit vinculin (1:1000) Cell Signaling Technology Cat. # 13901 Anti-rabbit GAPDH (1:1000) Cell Signaling Technology Cat. # 5174 Anti-rabbit phopho -NF-kB p65 (1:1000) Cell Signaling Technology Cat. # 3033S Anti-rabbit PGC1 -a (1:250) Cell Signaling Technology Cat. # 2178S Anti-rabbit fibronectin (1:100 – WB, 1:50 – IHC) Abcam Cat. # ab2413 Anti-rabbit type IV collagen (1:50 – IHC) Abcam Cat. # ab6586 Anti-goat type IV collagen (1:1000) |